=> Bootstrap dependency digest>=20211023: found digest-20220214
===> Building for bowtie2-2.5.2
clang++  -DCOMPILER_OPTIONS="\" -pipe -Os -I/opt/pkg/include/python3.11 -I/opt/pkg/include -std=c++11 -DPOPCNT_CAPABILITY -DNO_SPINLOCK -DWITH_QUEUELOCK=1\"" -pipe -Os -I/opt/pkg/include/python3.11 -I/opt/pkg/include -std=c++11 -DPOPCNT_CAPABILITY -DNO_SPINLOCK -DWITH_QUEUELOCK=1 \
	-fno-strict-aliasing -DBOWTIE2_VERSION="\"`cat BOWTIE2_VERSION`\"" -DBUILD_HOST="\"${HOSTNAME:-`hostname`}\"" -DBUILD_TIME="\"`date -u`\"" -DCOMPILER_VERSION="\"`clang++ -v 2>&1 | tail -1`\"" -D_LARGEFILE_SOURCE -D_FILE_OFFSET_BITS=64 -D_GNU_SOURCE  -DBOWTIE_MM  -DBOWTIE2 -DNDEBUG -Wall \
	-I/opt/pkg/include/python3.11 -I/opt/pkg/include -I third_party \
	-o bowtie2-build-s bt2_build.cpp \
	ccnt_lut.cpp ref_read.cpp alphabet.cpp shmem.cpp edit.cpp bt2_locks.cpp bt2_idx.cpp bt2_io.cpp bt2_util.cpp reference.cpp ds.cpp multikey_qsort.cpp limit.cpp random_source.cpp diff_sample.cpp bowtie_build_main.cpp \
	-L/opt/pkg/lib -lpthread -lz
In file included from bt2_build.cpp:28:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
In file included from ./diff_sample.h:27:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from bt2_build.cpp:28:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
In file included from bt2_build.cpp:28:
In file included from ./bt2_idx.h:60:
In file included from ./processor_support.h:17:
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:130:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:15: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:19: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                         ^~
                         %w1
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:22: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                            ^~
                            %w1
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:26: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                                ^~
                                %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:19: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                         ^~
                         %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:22: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                            ^~
                            %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:133:15: note: use constraint modifier "w"
           "push{l}\t%0\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:136:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:162:3: error: invalid output constraint '=a' in asm
  __cpuid (__ext, __eax, __ebx, __ecx, __edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
third_party/cpuid.h:185:3: error: invalid output constraint '=a' in asm
  __cpuid (__level, *__eax, *__ebx, *__ecx, *__edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
13 warnings and 2 errors generated.
In file included from ref_read.cpp:20:
In file included from ./ref_read.h:33:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
In file included from edit.cpp:21:
In file included from ./edit.h:29:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
In file included from bt2_idx.cpp:25:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
In file included from ./diff_sample.h:27:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from bt2_idx.cpp:25:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
In file included from bt2_idx.cpp:25:
In file included from ./bt2_idx.h:60:
In file included from ./processor_support.h:17:
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:130:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:15: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:19: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                         ^~
                         %w1
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:22: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                            ^~
                            %w1
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:26: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                                ^~
                                %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:19: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                         ^~
                         %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:22: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                            ^~
                            %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:133:15: note: use constraint modifier "w"
           "push{l}\t%0\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:136:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:162:3: error: invalid output constraint '=a' in asm
  __cpuid (__ext, __eax, __ebx, __ecx, __edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
third_party/cpuid.h:185:3: error: invalid output constraint '=a' in asm
  __cpuid (__level, *__eax, *__ebx, *__ecx, *__edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
13 warnings and 2 errors generated.
In file included from bt2_io.cpp:25:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
In file included from ./diff_sample.h:27:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from bt2_io.cpp:25:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
In file included from bt2_io.cpp:25:
In file included from ./bt2_idx.h:60:
In file included from ./processor_support.h:17:
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:130:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:15: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:19: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                         ^~
                         %w1
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:22: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                            ^~
                            %w1
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:26: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                                ^~
                                %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:19: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                         ^~
                         %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:22: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                            ^~
                            %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:133:15: note: use constraint modifier "w"
           "push{l}\t%0\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:136:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:162:3: error: invalid output constraint '=a' in asm
  __cpuid (__ext, __eax, __ebx, __ecx, __edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
third_party/cpuid.h:185:3: error: invalid output constraint '=a' in asm
  __cpuid (__level, *__eax, *__ebx, *__ecx, *__edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
13 warnings and 2 errors generated.
In file included from bt2_util.cpp:26:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
In file included from ./diff_sample.h:27:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from bt2_util.cpp:26:
In file included from ./bt2_idx.h:42:
In file included from ./blockwise_sa.h:41:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
In file included from bt2_util.cpp:26:
In file included from ./bt2_idx.h:60:
In file included from ./processor_support.h:17:
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:130:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:15: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:19: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                         ^~
                         %w1
third_party/cpuid.h:138:29: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                                   ^
third_party/cpuid.h:131:22: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                            ^~
                            %w1
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:131:26: note: use constraint modifier "w"
           "mov{l}\t{%0, %1|%1, %0}\n\t"
                                ^~
                                %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:19: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                         ^~
                         %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:132:22: note: use constraint modifier "w"
           "xor{l}\t{%2, %0|%0, %2}\n\t"
                            ^~
                            %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:133:15: note: use constraint modifier "w"
           "push{l}\t%0\n\t"
                     ^~
                     %w0
third_party/cpuid.h:138:14: warning: value size does not match register size specified by the constraint and modifier [-Wasm-operand-widths]
           : "=&r" (__eax), "=&r" (__ebx)
                    ^
third_party/cpuid.h:136:14: note: use constraint modifier "w"
           "pop{l}\t%0\n\t"
                    ^~
                    %w0
third_party/cpuid.h:162:3: error: invalid output constraint '=a' in asm
  __cpuid (__ext, __eax, __ebx, __ecx, __edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
third_party/cpuid.h:185:3: error: invalid output constraint '=a' in asm
  __cpuid (__level, *__eax, *__ebx, *__ecx, *__edx);
  ^
third_party/cpuid.h:104:7: note: expanded from macro '__cpuid'
           : "=a" (a), "=b" (b), "=c" (c), "=d" (d)     \
             ^
bt2_util.cpp:175:13: warning: variable 'restOff' set but not used [-Wunused-but-set-variable]
        TIndexOffU restOff = 0;
                   ^
14 warnings and 2 errors generated.
In file included from reference.cpp:22:
In file included from ./reference.h:32:
In file included from ./ref_read.h:33:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
In file included from ds.cpp:20:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
In file included from multikey_qsort.cpp:20:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from multikey_qsort.cpp:20:
In file included from ./multikey_qsort.h:27:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
4 warnings generated.
In file included from random_source.cpp:21:
In file included from ./random_util.h:25:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
In file included from diff_sample.cpp:20:
In file included from ./diff_sample.h:27:
In file included from ./multikey_qsort.h:24:
In file included from ./sequence_io.h:28:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
In file included from diff_sample.cpp:20:
./diff_sample.h:391:9: warning: variable 'amapEnts' set but not used [-Wunused-but-set-variable]
        size_t amapEnts = 1;
               ^
./diff_sample.h:574:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
./diff_sample.h:645:13: warning: variable 'added' set but not used [-Wunused-but-set-variable]
        TIndexOffU added = 0;
                   ^
4 warnings generated.
In file included from bowtie_build_main.cpp:25:
./ds.h:4078:8: warning: variable 'thru' set but not used [-Wunused-but-set-variable]
                        int thru = 0;
                            ^
1 warning generated.
gmake: *** [Makefile:329: bowtie2-build-s] Error 1
*** Error code 2

Stop.
bmake[1]: stopped in /Volumes/data/jenkins/workspace/pkgsrc-macos11-trunk-arm64/biology/bowtie2
*** Error code 1

Stop.
bmake: stopped in /Volumes/data/jenkins/workspace/pkgsrc-macos11-trunk-arm64/biology/bowtie2